RT  - Journal Article
A1  - Roy, Sukumar
A1  - Sultana, Mst. Tahsin
A1  - Rahman, Aminur
A1  - Hossain, Md. Shohorab
A1  - Binduraz, Bissas
A1  - Hanna, Tasnim
A1  - Abir, Md. Amirul Islam
A1  - Rahaman, Mahbuba
A1  - Abdullah-Al-Mamun, Md.
A1  - Roy, Monish Kumar
A1  - Cho, Kye Man
A1  - Haque, Md. Azizul
T1  - Genomic and In Silico Characterization of Acinetobacter sp. strain HSTU-Asm16 with Plant Growth-Promoting Traits and Diazinon Biodegradation Potential
JF  - Journal of Biosciences and Public Health
JO  - J. Biosci. Public Health
YR  - 2026
VO  - 2
IS  - 1
SP  - 68-95
DO  - 10.5455/JBPH.2026.02
AB  - Endophytic bacteria with combined plant growth-promoting (PGP) and pesticide biodegradation capacities offer sustainable agroecosystem management. This study reports the isolation, biochemical characterization, whole-genome sequencing, and in silico functional analysis of an endophytic bacterium, Acinetobacter sp. strain HSTU-Asm16, isolated from rice (Oryza sativa L.). Biochemical assays show catalase, oxidase, and citrate utilization; carbohydrate fermentation; and a suite of extracellular hydrolases consistent with plant-associated metabolism. The draft genome (~3.94 Mb) was annotated using the NCBI PGAP pipeline and analyzed for phylogenetic placement, including phylogenomics, average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH), pangenome assessment, and synteny analysis. Genome taxonomy placed HSTU-Asm16 within the Acinetobacter soli clade, confirming its status as a genomically distinct strain rather than a novel species. The identified genes are used in plant growth promotion (IAA, siderophore biosynthesis, ACC deaminase, and phosphate metabolism), stress tolerance (heat-/cold-shock proteins and heavy-metal resistance), and a complement of putative organophosphate-degrading enzymes (carboxylesterases, phosphotriesterases, amidohydrolases, and opd-like sequences). The genome encodes nif-associated and isc-related iron-sulfur cluster assembly genes, including nifS, nifU, iscU, and iscA, are involved in Fe–S protein maturation rather than canonical nitrogen fixation. Molecular docking with representative organophosphate ligands showed plausible substrate active site interactions for several hydrolases. The biochemical, genomic, and in silico evidence indicates Acinetobacter sp. HSTU-Asm16 is a promising plant-associated bacterium with dual potential for plant growth promotion and organophosphate pesticide bioremediation in rice farming.
K1  - Acinetobacter sp., Endophytic bacteria, plant growth–promoting traits, genomic analysis, organophosphorus hydrolase, Catalytic triads, pesticide biodegradation
SN  - 3104-8749
PB  - 4Green Research Society
LA  - English
LK  - https://www.jbph.org/article/details/genomic-and-in-silico-characterization-of-acinetobacter-sp-strain-hstu-asm16-with-plant-growth-promoting-traits-and-diazinon-biodegradation-potential