RT  - Journal Article
A1  - Binduraz, Bissas
A1  - Roy, Sukumar
A1  - Abdullah-Al-Mamun, Md.
A1  - Hossain, Md. Shohorab
A1  - Rahman, Aminur
A1  - Abir, Md. Amirul Islam
A1  - Sultana, Mst. Tahsin
A1  - Ghosh, Sibdas
A1  - Mortuza, Md. Golam
A1  - Cho, Kye Man
A1  - Haque, Md. Azizul
T1  - Unlocking Genomics of Multifunctional Endophytic Strain Citrobacter sp. HSTU-ABk15 from Rice Focusing Pesticide Bioremediation and Plant Health
JF  - Journal of Biosciences and Public Health
JO  - J. Biosci. Public Health
YR  - 2026
VO  - 2
IS  - 1
SP  - 96-123
DO  - 10.5455/JBPH.2026.03
AB  - Endophytic bacteria represent a promising biological solution for sustainable agriculture and pesticide bioremediation. This study reports the isolation and comprehensive characterization of the novel endophytic bacterial strain Citrobacter sp. HSTU-ABk15 from Oryza sativa L. tissues exposed to pesticide stress. Integrated biochemical, genomic, and in silico analyses revealed their dual functionality in chlorpyrifos degradation and plant growth promotion. The genome sequence analysis (ANI, dDDH, pangenomics, Progressive Mauve, and phylogenetic analysis) confirmed the strains belong to the Citrobacter species. The isolates exhibited phosphate solubilization, indole-3-acetic acid (IAA) production, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing genes in their annotated genome. Genome annotation identified organophosphate-degrading genes (opd, phn, ampD, pepD), suggesting potential for pesticide degradation. In silico docking analyses validated strong interactions (–6.5 to –8.0 kcal·mol⁻¹) between key enzymes (AmpD, PepE, GlpQ, carboxylesterase, amidohydrolase, and phosphonatase) and cypermethrin, diazinon, and crotoxiphos. Phylogenomic analyses (ANI and dDDH) confirmed their distinct taxonomic positions, indicating functionally distinct endophytic strains. Citrobacter sp. strain HSTU-ABk15 as a genetically robust, multifunctional endophyte with significant potential for eco-friendly pesticide remediation and sustainable rice cultivation.
K1  - Endophytic bacteria, plant growth promotion, Citrobacter sp., In-silico bioremediation, organophosphate-degrading genes, genomic analysis
SN  - 3104-8749
PB  - 4Green Research Society
LA  - English
LK  - https://www.jbph.org/article/details/unlocking-genomics-of-multifunctional-endophytic-strain-citrobacter-sp-hstu-abk15-from-rice-focusing-pesticide-bioremediation-and-plant-health